Description
Study of the reconstituted in vitro human PLK1:CENP-U complex with PBD fused N-terminally to maltose-binding-protein (MBPPBD) and residues 58-114 of CENP-U fused N-terminally to MBP (MBPCENP-U58-114). To phosphorylate T78 and T98 of MBPCENP-U58-114, we added catalytic amounts (typically 1:30 molar ratio) of active CDK1 and PLK1 kinase. Either 1) pre-phosphorylating MBPCENP-U58-114 with both kinases before adding MBPPBD for
complex formation (pre-phosphorylation method); or 2) mixing MBPCENP-U58-114 and MBPPBD
with both kinases for simultaneous phosphorylation and complex formation (co-mixing method) were tested.
[doi:10.25345/C5QJ78C0F]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: phosphorylation ; proteomics ; CENP-U ; DatasetType:Proteomics
Contact
Principal Investigators:
(in alphabetical order)
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Petra Janning, MPI of Molecular Physiology, Germany
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janning
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Publications
Long Ren, Arianna Esposito-Verza, Raphael Gasper, Marion E. Pesenti, Petra Janning, Franziska Müller, Carolin Koerner, Petra Geue, Sabine Wohlgemuth, Ingrid R. Vetter & Andrea Musacchio.
Molecular anatomy of PLK1 master docking motifs.
Nat Commun 17, 4228 (2026). https://doi.org/10.1038/s41467-026-73038-8.
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| Identification Results |
Proteins (Human, Remapped):
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Proteins (Reported):
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Number of distinct conditions across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct condition labels are counted across all files submitted in the "Metadata" category
having a "Condition" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct replicate labels are counted across all files submitted in the "Metadata" category
having a "BioReplicate" or "Replicate" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses)
associated with this dataset.
The technical replicate count is defined as the maximum number of times any one distinct
combination of condition and biological replicate was analyzed across all files submitted in the
"Metadata" category. In the case of fractionated experiments, only the first fraction is
considered.
"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically
remapped by MassIVE to proteins in the
SwissProt
human reference database.
"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and
reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original
submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported
across all analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all
analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison
across all analyses (original submission and reanalyses) associated with this dataset.
A protein is differentially abundant if its change in abundance across conditions is found
to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated
with statistical tests for differential abundance.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE.
It has been imported to MassIVE for reanalysis purposes, so its spectra data here may
consist solely of processed peak lists suitable for reanalysis with most software.