MassIVE MSV000093857

Partial Public PXD048516

Pla2g12b binding proteins in Zebrafish

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Description

We injected larvae with the wild-type rescue plasmid described above, and performed co-immunoprecipitation and mass spectrometry (co-IP/MS) against the highly specific 3x-FLAG epitope linked to Pla2g12b. The top five most abundant proteins included the core components of the TRL assembly pathway (ApoB and the two Subunits of Mtp (Mttp and Pdi/P4hb)), as well as various chaperone proteins . These interactions suggest that Pla2g12b participates directly in TRL biogenesis, and may promote efficient channeling of lipids to ApoB by linking it more tightly to the transfer protein Mtp. [doi:10.25345/C5RB6WC8M] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Pla2g12b

Contact

Principal Investigators:
(in alphabetical order) 		Steve Farber, Johns Hopkins University, USA
Submitting User: syjung
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