MassIVE MSV000094085

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Screen for proteins or peptides in human tears that are synergistic with N-104

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Description

It is evident that the effectiveness of N-104 decreases with increasing osmolarity. To investigate potential synergies, we exposed pre-cleared human tears in PBS to N-104 and a negative control, C-95, using affinity columns. After thorough PBS washing, eluants with 500 mM KCl were analyzed using tandem mass spectrometry. Specificity was examined by subtracting proteins interacting with C-95 from those bound to N-104. We identified affinity with 10 proteins known for antimicrobial activity, with the GKY20 (GKYGFYTHVFRLKKWIQKVI) peptide of thrombin being particularly supported by the literature. [doi:10.25345/C5DV1D025] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: lacritin ; N-104 ; human basal tears ; thrombin

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Principal Investigators:
(in alphabetical order) 		Gordon W. Laurie, Department of Cell Biology, University of Virginia, USA
Submitting User: anwaruddin
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