MassIVE MSV000097723

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Metabolomic analysis of stems of switchgrass lignin mutants under drought stress

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Description

This switchgrass project aims to identify metabolic consequences of lignin manipulation and impact on the responses to drought stress in switchgrass. Here we examine elongating internodes of six switchgrass lignin biosynthesis-altered transgenic and mutant and their isogenic wild-type negative segregant lines. Studies in the eudicot, Arabidopsis, have revealed metabolic and transcriptional differences in lignin biosynthesis mutants that appear to relate to feedback mechanisms for plants to compensate for lignin pathway disruption. Specifically, disruption of core lignin biosynthesis gene expression leads to increases in expression of cell wall-related pathways; whereas, disruption of downstream S- and G-lignin specific genes leads to down-regulation of lignin biosynthesis. This experiment allows us to test the generality of this observation in a bioenergy-relevant species. While we generally expect the same patterns, progress revealing differences in grass lignin biosynthesis pathways relative to eudicots suggests that we might also uncover differences. The work (proposal:https://doi.org/10.46936/10.25585/60000468) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231. [doi:10.25345/C54T6FG1F] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: switchgrass ; drought stress ; lignin biosynthesis ; DatasetType:Metabolomics

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Principal Investigators:
(in alphabetical order) 		Mariela Monteoliva, University of Oklahoma, United States
Submitting User: bpbowen
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