MassIVE MSV000099423

Partial Public PXD069213

Anti-C-RAF IP-MS in MG-treated NCI-H226 cells

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Description

NCI-H226 cells in 100 mm dishes were treated with 10 uM of compounds 22 or 23 or DMSO at equal volume (final [DMSO] = 0.02%) for 24 h in triplicates for each condition (n = 3). Soluble lysates proceeded to anti-endogenous CRAF IP-MS with anti-CRAF antibody (BD Biosciences 610152; incubated at 4C overnight) and protein A/G beads (Pierce 88802; incubated at RT for 1 h), and on-bead digestion with PreOmics sample cleanup kit. 200 ng of peptides were quantified and injected onto Orbitrap Eclipse with 60 min gradient using DIA. [doi:10.25345/C5542JN5R] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Molecular glue ; C-RAF ; 14-3-3 ; SFN ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Michelle Arkin, UCSF, USA
Submitting User: shuluo
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Identification Results
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Quantification Results
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
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