MassIVE MSV000096722

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Cyanobacterial circadian regulation enhances bioproduction under subjective nighttime through rewiring of carbon partitioning dynamics, redox balance orchestration, and cell cycle modulation

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Description

GC-MS extracellular metabolite profiling of circadian clock regulation impacts of carbon partitioning between storage, growth, and product synthesis in Synechococcus elongatus PCC 7942 for enhanced bioproduction strategy investigation. Metabolomic targeted analysis of PCC 7942 light-dark cycle cultures transitioned to constant light revealed distinct temporal patterns in sucrose production. Culture samples (8 mL) were collected at 0, 0.5, 1, 2, 4, 6, and 8 hours then dried under speed vacuum and dissolved in pyridine and chemically derivatized by methoxyamination and trimethylsilylation for extracellular sucrose analysis. Data was analyzed using Agilent Mass Hunter. Culture samples (8 mL) were collected at 0, 0.5, 1, 2, 4, 6, and 8 hours then dried under speed vacuum and dissolved in pyridine and chemically derivatized by methoxyamination and trimethylsilylation for extracellular sucrose analysis. [doi:10.25345/C59K4652C] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: circadian regulation ; cyanobacteria ; metabolomics ; sucrose quantification ; DatasetType:Metabolomics

Contact

Principal Investigators:
(in alphabetical order) 		Pavlo Bohutskyi, Pacific Northwest National Laboratory, United States
Submitting User: alchemistmatt

Publications

Lindsey N Anderson, Zachary D Johnson, Katrina M Waters, and Pavlo Bohutskyi.
PPI DataHub Project Data Package: S. elongatus PCC 7942 Circadian Control Bioproduction Metabolomics (PB-DP5).
doi: 10.25584/2510499.

Gilliam A, Sadler NC, Li X, Garcia M, Johnson Z, Veli?kovi? M, Kim YM, Feng S, Qian WJ, Cheung MS, Bohutskyi P.
Cyanobacterial circadian regulation enhances bioproduction under subjective nighttime through rewiring of carbon partitioning dynamics, redox balance orchestration, and cell cycle modulation.
Microb Cell Fact. 2025 Mar 8;24(1):56. Epub 2025 Mar 8.

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