MassIVE MSV000081198

Partial Public

Integrated in vivo multi-omics identifies p21-activated kinase signaling as a driver of colitis

Description

Proteome and phosphopoteome mappings of colon samples from a inflammatory bowel disease mouse model. Two TMT-sets for proteome mappings were generated (set1 and set2). The labeling scheme is: sample 410 (set1, 127n), sample 411 (set1, 128c), sample 421 (set1, 129c), sample 422 (set1, 129n), sample 426 (set2, 128c), sample 430 (set2, 128n), sample 431 (set2, 129c), sample 432 (set2, 129n). One TMT-set was run for phosphopeptide mapping. The labeling scheme is: sample 410 (127n), sample 411 (127c), sample 421 (128n), sample 422 (128c), sample 426 (129n), sample 430 (129c), sample 431 (130n), sample 432 (130c). [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: proteome, phosphoproteome, TMT, mouse model, inflammatory bowel disease

Contact

Principal Investigators:
(in alphabetical order)
Kevin M. Haigis, Cancer Research Institute and Department of Medicine, Beth-Israel Deaconess Medical Center, Boston, Massachusetts, USA, USA
Submitting User: whaas
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Experimental Design
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Identification Results
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Quantification Results
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.