MassIVE MSV000094638

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NCI-H226 TEAD4 FLAG AP-MS profiling

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Description

The goal of this experiment is to identify changes in TEAD4 protein interactions upon treatment with TEAD small molecule inhibitors. NCI-H226 cells were treated for 24h with DMSO vehicle control or a TEAD inhibitor, GNE-7883 or Compound 2. Immunoprecipitation of TEAD4 was performed and the resulting protein samples were processed for tandem mass tag (TMT) labeling and quantitative proteomic analysis. Pooled TMT-labeled samples were fractionated into six fractions and the experiment was performed in triplicate. For further experimental details, see the Materials and Methods section in the manuscript. [doi:10.25345/C5ZW19402] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: APMS ; TMT ; TEAD4

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Principal Investigators:
(in alphabetical order) 		Jennie Lill, Genentech Inc, USA
Submitting User: mnchoi
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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