MassIVE MSV000090546

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Simultaneous Screening and Quantitation of Human Milk Oligosaccharides by Liquid Chromatography – Mass Spectrometry

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Description

An analytical method which enables the simultaneous quantitation of seven abundant HMOs (i.e., 2-FL, LNT, LNFP I, LNDFH I, 3-SL, DSLNT, and 6-SL) and the simultaneous screening of a wide variety of HMOs is presented. First, sample preparation included an SPE purification step that enabled a quantitative extraction of HMOs. Then, isolated HMOs are analyzed using hydrophilic interaction liquid chromatography (HILIC) coupled to a Q-Exactive Plus Mass Spectrometer from Thermofisher operating in polarity switching mode with a total runtime of 32.5 minutes and with help of an inclusion list constructed from the information contained in the NIST Milk Oligosaccharide MS Library. Three complementary data processing strategies were employed: (1) HMO quantitation, (2) screening, and (3) molecular networking. [doi:10.25345/C5G15TG47] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: human milk ; human milk oligosaccharides ; pasteurization ; LC-MS ; molecular networking

Contact

Principal Investigators:
(in alphabetical order) 		Isabel Ten-Domenech, Health Research Institute La Fe, Spain
Submitting User: tendoi

Publications

Víctor Navarro-Esteve, Anna Zöchner, Marta Roca, Anna Parra-Llorca, Alba Moreno-Giménez, Laura Campos-Berga, María Jesús Vaya, Máximo Vento, Pilar Sáenz González, María Gormaz, Isabel Ten-Doménech, Julia Kuligowski, Guillermo Quintás.
Simultaneous screening and quantitation of human milk oligosaccharides by liquid chromatography—Mass spectrometry.
Carbohydrate Polymer Technologies and Applications. 2025; 9: 100644. doi: 10.1016/j.carpta.2024.100644.

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