MassIVE MSV000084655

Partial Public PXD016592

gender-related secretome of Plasmodium berghei sexual stages

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Description

In this study, we applied a proteomic approach to identify proteins differentially released/secreted by female and male gametocytes of the rodent Plasmodium berghei activated to form gametes. We compared secreted molecules of the wild type gametocytes with those of a transgenic line defective in male gamete maturation and egress. This enabled us to provide a comprehensive dataset of egress-related molecules and their gender specificity. Using specific antibodies, we validated eleven candidate molecules, predicted as either gender-specific or common to both male and female gametocytes. All of them localize to punctuate, vesicle-like structures that relocate to cell periphery upon activation, but only three of them localize to the gametocyte-specific secretory vesicles named osmiophilic bodies. Our results confirm that the egress process involves a tightly coordinated secretory apparatus that includes different types of vesicles and may put the basis for functional studies aimed at designing novel transmission-blocking molecules. [doi:10.25345/C5N38G] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: SECRETOME, VESICLES, PLASMODUM BERGHEI, GAMETOCYTES

Contact

Principal Investigators:
(in alphabetical order) 		FEDERICA FRATINI, ISS, Italia
Submitting User: federica_fratini

Publications

Grasso F, Mochi S, Fratini F, Olivieri A, Currà C, Siden Kiamos I, Deligianni E, Birago C, Picci L, Pizzi E, Pace T, Ponzi M.
A Comprehensive Gender-related Secretome of Plasmodium berghei Sexual Stages.
Grasso F, Mochi S, Fratini F et al., Mol Cell Proteomics. 2020 Dec;19(12):1986-1997. doi: 10.1074/mcp.RA120.002212.

Grasso F, Fratini F, Albanese TG, Mochi S, Ciardo M, Pace T, Ponzi M, Pizzi E, Olivieri A.
Identification and preliminary characterization of Plasmodium falciparum proteins secreted upon gamete formation.
Grasso F, Fratini F, et al., Sci Rep. 2022 Jun 10;12(1):9592. doi: 10.1038/s41598-022-13415-7.

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