MassIVE MSV000099804

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On the utility of infrared photoactivation for native top-down and complex-down Orbitrap mass spectrometry of soluble proteoform complexes

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Description

We implemented infrared (IR) activation for the analysis of soluble multi proteoform complexes (MPCs): alcohol dehydrogenase (ADH; 147 kDa tetramer), enolase (96 kDa dimer), and pyruvate kinase (PK; 232 kDa tetramer). IR photons were used to induce infrared multiphoton dissociation (IRMPD) and to enhance electron-based fragmentation via activated-ion ETD (AI-ETD), and performance was benchmarked against higher-energy collisional dissociation (HCD). [doi:10.25345/C5639KJ7D] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: orbitrap ; native top down (nTD) ; complex down (CxD) ; photoactivation ; electron transfer dissociation (ETD) ; multi proteoform complex (MPC) ; DatasetType:Proteomics

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Principal Investigators:
(in alphabetical order) 		Luca Fornelli, University of Oklahoma, United States
Submitting User: Fornelli_Lab
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