MassIVE MSV000094738

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GNPS - Lipidomics of homeoviscous adaptation to low temperatures in Staphylococcus aureus utilizing exogenous straight-chain unsaturated fatty acids over biosynthesized endogenous branched-chain fatty acids

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Description

It is well established that Staphylococcus aureus can incorporate exogenous straight-chain unsaturated fatty acids (SCUFAs) into membrane phospho- and glyco-lipids from various sources in supplemented culture media, and when growing in vivo in an infection. Given the enhancement of membrane fluidity when oleic acid (C18:1(9z)) is incorporated into lipids, we were prompted to examine the effect of medium supplementation with C18:1(9z) on growth at low temperatures. C18:1(9z) supported the growth of a cold-sensitive, branched-chain fatty acid (BCFA)-deficient mutant at 12C. Interestingly, we found similar results in the BCFA-sufficient parental strain. We show that incorporation of C18:1(9z) and its elongation product C20:1(9z) into membrane lipids was required for growth stimulation and relied on a functional FakAB incorporation system. Lipidomics analysis of the phosphatidylglycerol (PG) and diglycosyldiacylglycerol (DGDG) lipid classes revealed major impacts of C18:1(9z) and temperature on lipid species. Growth at 12C in the presence of C18:1(9z) also led to increased production of the carotenoid pigment staphyloxanthin; however, this was not an obligatory requirement for cold adaptation. Enhancement of growth by C18:1(9z) is an example of homeoviscous adaptation to low temperatures utilizing an exogenous fatty acid. This may be significant in the growth of S. aureus at low temperatures in foods that commonly contain C18:1(9z) and other SCUFAs in various forms. [doi:10.25345/C5251FW9M] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: lipids, lipidomics, staphylococcus aureus, oleic acid, low temperature

Contact

Principal Investigators:
(in alphabetical order) 		Brian J. Wilkinson, Illinois State University, USA
Kelly M. Hines, University of Georgia, USA
Submitting User: kmhines5

Publications

Barbarek SC, Shah R, Paul S, Alvarado G, Appala K, Phillips C, Henderson EC, Strandquist ET, Pokorny A, Singh VK, Gatto C, Dahl J-U, Hines KM, Wilkinson BJ.
Lipidomics of homeoviscous adaptation to low temperatures in Staphylococcus aureus utilizing exogenous straight-chain unsaturated fatty acids.
J Bacteriol. 2024 Jul 25;206(7):e0018724. Epub 2024 Jul 2.

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