MassIVE MSV000089917

Complete Public PXD035387

DSSO Crosslinking of Intact Tetrahymena Thermophila Cilia

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Description

The MS-cleavable crosslinker, DSSO, was added to cilia isolated from Tetrahymena thermophila. After digestion crosslinked peptides were enriched by size exclusion chromatography. Data were collected using an MS2/MS3 method. Analysis was done using the XlinkX node of PD2.3 and a fasta file generated from standard MS1/MS2 analysis of the crosslinked samples. [doi:10.25345/C55M62B6R] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: DSSO, XL-MS, Tetrahymena thermophila, cilia

Contact

Principal Investigators:
(in alphabetical order) 		Edward Marcotte, University of Texas-Austin, United States
Submitting User: OpheliaP

Publications

McCafferty CL, Pennington EL, Papoulas O, Taylor DW, Marcotte EM.
Does AlphaFold2 model proteins' intracellular conformations? An experimental test using cross-linking mass spectrometry of endogenous ciliary proteins.
Commun Biol. 2023 Apr 15;6(1):421. Epub 2023 Apr 15.

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