MassIVE MSV000097748

Partial Public

AIMP3 Maintains Cardiac Homeostasis by Regulating the Editing Activity of Methionyl-tRNA Synthetase

Subscribe Comment Convert Spectra Reanalyze Spectra Add Reanalysis

Description

Ventricles from Ctrl (n=4, labelled as C1-C4) and A3cKO (n=4, labelled as B1-B4) were excised after 10 days of 1st tamoxifen I.P., and lysates were prepared in lysis buffer (50 mM Tris-HCl, pH 7.6, 150 mM NaCl, 0.5% Na deoxycholate, 1% SDS, 1 mM EDTA without protease inhibitors) using electronic hand homogenizer. Lysates were sonicated in water bath sonicator for 10 min at 4C and centrifuged at 20,000g for 20 min at 4C. Protein concentrations were measured using the BCA method from the supernatants, and 100 microG total protein samples were submitted to the OSU CCIC Mass Spectrometry and Proteomics (MSP) Facility. LC-MS/MS was performed using the label-free method, and results were analyzed in Mascot software. [doi:10.25345/C51Z4255G] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Cardiac hypertrophy ; tRNA multisynthetase complex ; AIMP3 ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Federica Accornero, Brown University, USA
Submitting User: adas

Publications

Anindhya S. Das, Charles P. Rabolli, Colton R. Martens, Han-Kai Jiang, Yingshen Zhang, Aubree A. Zimmer, Kevin Lin, Kedryn K. Baskin, Juan D. Alfonzo & Federica Accornero.
AIMP3 maintains cardiac homeostasis by regulating the editing activity of methionyl-tRNA synthetase.
Nat Cardiovasc Res (2025). https://doi.org/10.1038/s44161-025-00670-w.

Number of Files:
Total Size:
Spectra:
Subscribers:
 
Owner Reanalyses
Experimental Design
    Conditions:
    Biological Replicates:
    Technical Replicates:
 
Identification Results
    Proteins (Human, Remapped):
    Proteins (Reported):
    Peptides:
    Variant Peptides:
    PSMs:
 
Quantification Results
    Differential Proteins:
    Quantified Proteins:
 
Browse Dataset Files
 
FTP Download Link (click to copy):

Species

Instrument

Modifications

- Dataset Reanalyses

+ Dataset History

Click here to queue conversion of this dataset's submitted spectrum files to open formats (e.g. mzML). This process may take some time.

When complete, the converted files will be available in the "ccms_peak" subdirectory of the dataset's FTP space (accessible via the "FTP Download" link to the right).
Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.