MassIVE MSV000086694

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The protein phosphatase PPM1A dephosphorylates and activates YAP to govern mammalian intestinal and liver regeneration

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Description

Nanoscale liquid chromatography coupled to tandem mass spectrometry analysis for protein identification, characterization and label-free quantification was performed by Phoenix National Proteomics Core. The samples for mass spectrometry analyses were from the in vitro phosphatase assays mentioned above, separated by SDS-PAGE, stained by coomassie blue to locate the YAP, and cut spanning YAP. Pryptic peptides were separated on a C18 column and analyzed by LTQ-Orbitrap Velos (Thermo). Proteins were identified using the search engine of the National Center for Biotechnology Information against the human or mouse RefSeq protein databases. [doi:10.25345/C5549V] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: PPM1A ; Hippo pathway ; YAP ; Phosphorylation

Contact

Principal Investigators:
(in alphabetical order) 		Pinglong Xu, Zhejiang university, China
Submitting User: Ruyuan
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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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