MassIVE MSV000095123

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GNPS - Multi-ROI, multi-condition, and multi-replicate metabolite MALDI imaging of mouse heart and pancreas

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Description

Mouse heart and pancreas tissues for imaging mass spectrometry were removed from animal organs, frozen on dry ice, and then stored at -80-degree C until analysis. 12-micrometer mouse heart tissues were sectioned using a Leica CM 3050S Research Cryostat (Leica Biosystems, Wetzlar, Germany) using -25-degree C chamber temperature and -23-degree C object temperature, prior to thaw mounting onto indium tin oxide-coated microscope slides (Delta Technologies, Loveland, CO, USA). A 10 mg/mL solution of 1,5-diaminonaphthalene (DAN) MALDI matrix layer was applied to the microscope slide using an HTX M5 TM Sprayer (HTX Technologies, LLC, Chapel Hill, NC, USA) with 0.1 mL/min flow rate over 4, 6, or 8 passes. MALDI imaging mass spectrometry was performed on a 7T solariX Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer equipped with a dynamically harmonized ParaCell XR (Bruker Daltonics, Bremen, Germany). Analysis was performed in negative ion mode from m/z 75 to 500 with a 1 megaword transient (~0.4 s ICR transient length). A 117 (+/-) 75 m/z continuous accumulation of selected ions (CASI) window was used to perform gas-phase enrichment of low m/z metabolites. The MALDI source is equipped with a Smartbeam II Nd:YAG MALDI laser and was used to sample at a pixel spacing of 200 micrometers in the x and y dimensions using 200 laser shots per pixel (minimum laser focus, 2 kHz frequency) with Smart Walk enabled [doi:10.25345/C59P2WH9S] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: mouse heart ; mouse pancreas ; MALDI ; imaging mass spectrometry ; mass spectrometry imaging

Contact

Principal Investigators:
(in alphabetical order) 		Boone M. Prentice, University of Florida, USA
Submitting User: yutinlin
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Identification Results
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GNPS content goes here (MSV000095123 [task=332dca24fe4b4fbdb8e1f3ae73c9544e])
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.