MassIVE MSV000096866

Complete Public PXD059869

Phosphoproteomics of RAPTOR1 and LST8-1 PUP-IT samples (inducible expression)

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Description

PUP-IT proximity labeling of Arabidopsis Target of Rapamycin Complex (TORC) subunits LST8-1 and RAPTOR1, compared against a GFP control, with beta-estradiol-inducible expression of FLAG::PUP(E). The FLAG-enriched samples were additionally enriched for phosphorylated peptides. The sample metadata, sequence database and fragpipe workflow are uploaded under the 'Metadata' tag. [doi:10.25345/C53B5WM32] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: PUP-IT ; Proximity labeling ; Target of Rapamycin ; TOR ; Phosphoproteomics ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Staffan Persson, Copenhagen University, Denmmark
Submitting User: leonardblaschek
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.