MassIVE MSV000085995

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Peptide profiling of laser microdissected human trophoblast subtypes during the second trimester

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Description

Human placental architecture is complex. Its surface epithelium, specialized for transport, forms by fusion of cytotrophoblast progenitors into multinucleated syncytiotrophoblasts. Near the uterine surface, these progenitors assume a different fate, becoming cancer like cells that invade its lining and blood vessels. The latter process physically connects the placenta to the mother and shunts uterine blood to the syncytiotrophoblasts. Isolation of trophoblast subtypes is technically challenging. Upon removal, syncytiotrophoblasts disintegrate and invasive cytotrophoblasts are admixed with uterine cells. We used laser capture to circumvent these obstacles. This enabled isolation of syncytiotrophoblasts and two subpopulations of invasive cytotrophoblasts; cell column and endovascular. Transcriptional profiling revealed numerous genes whose placental or trophoblast expression was not known, including neurotensin and C4ORF36. Using mass spectrometry, discovery of differentially expressed mRNAs was extended to the protein level. We also found that invasive cytotrophoblasts expressed cannabinoid receptor 1. Unexpectedly, screening agonists and antagonists showed signals from this receptor promote invasion. Together these results revealed novel gene expression patterns that translate to the protein level. Our data also suggested that endogenous and exogenous cannabinoids can affect human placental development. [doi:10.25345/C5XX87] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Human placenta, laser microdissection, DIRECTOR slides, spectral counting, negative binomial distribution

Contact

Principal Investigators:
(in alphabetical order) 		Susan Fisher, University of California San Francisco, United States of America
Submitting User: mjmgormley
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