This submission contains the mass spectrometry files for the manuscript by Samavarchi-Tehrani et al. that describes the use of lentiviral delivery of BirA*-tagged transgenes into HeLa, BJ and MEF cells or in comparison to stably integrated transgene in Flp-In T-REx HeLa cell lines. This submission contains the human files from a set of 56 raw MS files and associated peak lists and result files, acquired on a TripleTOF 6600 spectrometer (AB SCIEX, Concord, Ontario, Canada) operated in Data Dependent Acquisition mode. Samples were generated by Payman Samavarchi-Tehrani.
This massIVE submission contains the files for the mouse samples. Please see accompanying submission for the human samples.
Raw files are deposited here in MassIVE. For questions, please contact Anne-Claude Gingras (gingras@lunenfeld.ca) or Payman Samavarchi-Tehrani (payman@lunenfeld.ca).
Table 1 describes the composition of this dataset
Table 2 lists all the peptide identification evidence (as per iProphet)
Table 3 lists the SAINTexpress interactions
Internal reference from the Gingras lab ProHits implementation:
Project 112, Export versions VS4 (Viral BioID Benchmark)
SAINT Task ID: Flp-HeLa:3596; HeLa:3139; BJ:3140; MEF:3141
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Affinity purification ; BioID ; Proximity-dependent biotinylation ; Protein-protein interaction ; AP-MS ; Lentivirus
Principal Investigators: (in alphabetical order) |
Anne-Claude Gingras, LTRI, Canada |
Submitting User: | gingraslab |
Samavarchi-Tehrani P, Abdouni H, Samson R, Gingras AC.
A versatile lentiviral delivery toolkit for proximity-dependent biotinylation in diverse cell types.
Mol. Cell Proteomics. Epub 2018 Jul 10.
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Identification Results | ||
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Differential Proteins:
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Quantified Proteins:
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