MassIVE MSV000088585

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Graft Source-dependent Biomarkers for Risk of Chronic Graft-Versus-Host Disease and Death

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Description

We identify risk markers and graft specific markers at day 90 post-HCT (hematopoietic cell transplant). For this, we developed a 4-plex TMT plasma proteomic approach comparing day-90 samples from PB (peripheral-blood stem cells) graft recipients who developed cGVHD by day 180, PB graft recipients who did not develop cGVHD, BM (bone marrow) graft recipients who developed cGVHD by day 180, BM graft recipients who did not developed cGVHD. Five markers were candidate risk markers and selected to move to the validation phase. Using ELISA assays these 5 markers were measured along with nine previously identified cGVHD proteomic biomarkers (ST2, CXCL9, MMP3, OPN, CXCL10, soluble CD163, IL17, B-cell activating factor (BAFF), and C-C-motif-chemokine-15 (CCL15)16,23-29 in 335 recipients (from CTN0201 to test if they were markers of risk and graft-specific markers). Finally, we measured the six significant candidate risk markers in CTN0201 in 653 patients from the CTN1202 study, an independent contemporary cohort. [doi:10.25345/C5RW09] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Tandem mass tags, TMT, Graft-versus-host, GVHD, plasma, biomarker

Contact

Principal Investigators:
(in alphabetical order) 		Sophie Paczesny, Medical University of South Carolina, United States
Submitting User: FHproteomics
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