MassIVE MSV000093044

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Proteome analysis of epithelial-mesenchymal transition extracellular vesicles: exosomes, microparticles and shed midbody remnants

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Description

Cell-derived extracellular vesicles (EVs) are evolutionary-conserved secretory organelles that, based on their molecular composition, are important intercellular signaling regulators. At least three classes of circulating EVs are known based on mechanism of biogenesis: exosomes (sEVs/Exos), microparticles (lEVs/MPs) and shed midbody remnants (lEVs/sMB-Rs). Here we conducted large-scale purification of exosomes, MPs and sMBRs isolated from MDCK and 21D1 cells, and performed comprehensive proteomics analysis to differentiate these EV subtypes. Findings from this study suggests that sMBRs may hold crucial clues to the regulation of EMT and the involvement of different subtypes of EVs based on their dynamic proteome landscape in this process. [doi:10.25345/C5PN8XR7Q] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: extracellular vesicles ; proteomics ; subtypes ; shed midbody remnants ; epithelial-mesenchymal transition

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Principal Investigators:
(in alphabetical order) 		David Greening, Baker Heart & Diabetes Institute, Australia
Submitting User: dwgree
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
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