MassIVE MSV000094979

Partial Public PXD052996

Proteomic investigation of seed development under high nitrate supply in plants lacking Pyrroline-5-carboxylate dehydrogenase

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Description

Free proline is an amino acid known to have many functions in plants, particularly in stress adaptation and development. Its metabolism is well known, but its exact role in plant physiology has remained elusive. Proline catabolism is ensured by the sequential action of the enzymes Proline dehydrogenase (ProDH) and Pyrroline-5-carboxylate dehydrogenase (P5CDH). In this study, seed development in the p5cdh mutant was investigated under low and high nitrate supply. It was found that, in p5cdh mutants, embryo development was blocked at the transition between embryogenesis and the maturation phase. As a result, the whole maturation phase was disturbed, starting with a shorter photosynthetic phase, followed by a lower accumulation of storage compounds but a higher accumulation of proline when compared to wild-type embryos. Finally, at the end of seed development, mutant embryos appeared to be unable to tolerate desiccation and died during this final stage of development. This phenotype was strongly enhanced under high nitrate. Together with multiomics analysis, these data show that proline catabolism is important for redox homeostasis and energy supply during seed maturation. This dataset contains the respective proteomic data of the project. [doi:10.25345/C5X63BH1F] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Arabidopsis ; Seeds ; Seed development ; Pyrroline-5-carboxylate dehydrogenase ; high nitrate supply

Contact

Principal Investigators:
(in alphabetical order) 		Hans-Peter Braun, Plant Genetics, department plant proteomics, Germany
Nils Rugen, Leibniz University Hannover, Germany
Submitting User: Nils_2023

Publications

Dourmap C, Baud S, Eubel H, Rugen N, Crilat É, Beradocco S, Marnet N, Clément G, Tcherkez G, Planchais S, Cabassa C, Lebreton S, Bordenave-Jacquemin M, Maldiney R, Carol P, Braun HP, Masclaux-Daubresse C, Bouchereau A, Savouré A, Guivarc'h A.
Pyrroline-5-carboxylate dehydrogenase is a key actor of nitrogen metabolism in maturing seeds of Arabidopsis thaliana.
J Exp Bot. Epub 2026 Jan 12.

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