MassIVE MSV000094020

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Ubiquitin remnant motif profiling (Ubiquitomics) in VACV infected HeLa cells

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Description

HeLa cells were pretreated with MG132, inoculated with VACV-Cop at an MOI of 3 for 1 h, and posttreated with MG132 again. Cells were lysed and extracted proteins were digested to peptides using trypsin, and desalted peptides were subjected to automated diGly enrichment using the PTMScan ubiquitin remnant motif kit (Cell signaling) according to manufactuer's instructions. [doi:10.25345/C5TB0Z60P] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: ubiquitomics ; VACV ; ubiquitin ; ubiquitin remnant motif

Contact

Principal Investigators:
(in alphabetical order) 		Olivier Julien, University of Alberta, Canada
Robert Ingham, University of Alberta, Canada
Submitting User: julienlab
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Experimental Design
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Identification Results
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Quantification Results
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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