MassIVE MSV000078782

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Lambert_P82_VS4_Chromatin_BioID_APMS_12JUL2014

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Description

This submission contains the mass spectrometry files for the manuscript by Lambert et al. that describes the use of BioID for chromatin associated protein complexes. A detail comparison to a chromatin optimized AP-MS protocol was also performed. This submission contains 39 IDA files for the following baits: empty 3XFLAG control, GFP-BirA-FLAG control, NLS-BirA-FLAG control, H2B-BirA-FLAG, H3.1-BirA-FLAG, 3XFLAG-MED4, BirA-FLAG-MED4, MED20-3XFLAG, MED20-BirA-FLAG, 3XFLAG-MED23 and BirA-FLAG-MED23. Two biological replicates for every bait were analyzed by the standard Gingras lab BioID protocol and by a protocol optimized for chromatin associated baits (AP-MS). See the README file within "Methods and Protocols" and the accompanying File description. Additional details and files are found on prohits-web.lunenfeld.ca. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Anne-Claude Gingras
Submitting User: gingraslab

Publications

Lambert JP, Tucholska M, Go C, Knight JD, Gingras AC.
Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes.
J Proteomics. Epub 2014 Oct 2.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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