MassIVE MSV000085606

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Proteome and phosphoproteome of brassinolide treated Arabidopsis

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Description

We performed proteome and phosphoproteome profling of Arabidopsis plants to determine brassinosteroid responsive changes. Seeds were germinated and grown for 7 days on plates containing 1 uM brassinozole (BRZ) to eliminate any background BR signaling. on day 7 plants were treated with brassinolide (0 uM or 1 uM BL) for six different lengths of time (15min, 30min, 1hr, 2hr, 4hr, 8hr). Proteins were extracted and digested using the Phenol-FASP method described in https://doi.org/10.1002/pmic.201800220. Peptides were TMT labeled. Phosphopeptides were serially enriched using High-Select TiO2 and High-Select Fe-NTA kits. [doi:10.25345/C5S72T] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Arabidopsis ; brassinosteroid ; brassinolide

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Principal Investigators:
(in alphabetical order) 		Justin Walley, Iowa State University, USA
Submitting User: jwalley
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