MassIVE MSV000082812

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Comparison of Drosophila melanogaster adult and embryo proteome

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Description

Embryo and adult flies infected with Wolbachia pipientis or cured were lysed in a SDS containing buffer and digested with trypsin using in gel digestion. The resulting peptides were injected on a Sciex Triple-TOF 6600 in SWATH-MS mode. The data were analysed using Spectronaut 10 using the spectral library published in Fabre et al, Proteomics, 2017. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: SWATH-MS ; Development ; Drosophila melanogaster

Contact

Principal Investigators:
(in alphabetical order) 		Bertrand Fabre, Techinion, Israel
Submitting User: bertrandfabre31

Publications

Fabre B, Korona D, Lees JG, Lazar I, Livneh I, Brunet M, Orengo CA, Russell S, Lilley KS.
Comparison of Drosophila melanogaster embryo and adult proteome by SWATH-MS reveals differential regulation of protein synthesis, degradation machinery and metabolism modules.
J. Proteome Res. Epub 2019 May 14.

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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.