Description
Guarana samples were analyzed by LC-MS/MS ESI and tandem MS profiles were obtained.
100 mg of guarana powder were accurately weighted and suspended in 1.5 mL of the mixture of acetonitrile (HPLC grade) and TFA solution adjusted previously at pH 2.0. The mixture was sonicated in an USC 1800 ultrasound bath (Unique, Brazil) for 60 min, then centrifuged for 30 min at 13,400 rpm in a MiniSpin plus centrifuge (Eppendorf, Billerica, CA), and finally, the supernatant was separated in microtube (Eppendorf), evaporated, frozen and lyophilized.
The LC-MS methos was development was performed in coluna 5uPFP2;100A_15X2
Flx=0,1875 mL/minSSplit; P:971psi;F:30C; C=1mg/mL(MeOH/H2O_1:1_0.1%ACF);Inj=10uL;
A(H2O+0.1%ACF)/B(ACN+0,1%ACF) 0-60_8-22%; 60-62m_22-8% ; 62-70m _8%
injetor: MeOH/H2O_(0.1%ACF_1:1; Calib.:HCOONa 10mM_end
[doi:10.25345/C5MW28K2Q]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: guarana ; Paullinia cupana ; Amazonian plants
Contact
Principal Investigators:
(in alphabetical order)
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Josiel do Nascimento Amazonas, Universidade Federal do Amazonas, Brazil
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josiel
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GNPS content goes here (MSV000090891 [task=3f0e0dd14a364b77b588c955d55ae504])
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Number of distinct conditions across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct condition labels are counted across all files submitted in the "Metadata" category
having a "Condition" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct replicate labels are counted across all files submitted in the "Metadata" category
having a "BioReplicate" or "Replicate" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses)
associated with this dataset.
The technical replicate count is defined as the maximum number of times any one distinct
combination of condition and biological replicate was analyzed across all files submitted in the
"Metadata" category. In the case of fractionated experiments, only the first fraction is
considered.
"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically
remapped by MassIVE to proteins in the
SwissProt
human reference database.
"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and
reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original
submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported
across all analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all
analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison
across all analyses (original submission and reanalyses) associated with this dataset.
A protein is differentially abundant if its change in abundance across conditions is found
to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated
with statistical tests for differential abundance.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE.
It has been imported to MassIVE for reanalysis purposes, so its spectra data here may
consist solely of processed peak lists suitable for reanalysis with most software.