MassIVE MSV000080764

Imported Reanalysis Dataset Public PXD004668

Interactome of Nematostella GW182 in HEK293 cells

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Description

Here, we dissect the function of GW182 protein in the cnidarian Nematostella, separated by 600 million years from other Metazoa. Using cultured human cells, we show that Nematostella GW182 recruits the CCR4-NOT deadenylation complexes via its tryptophan-containing motifs, thereby inhibiting translation and promoting mRNA decay. Further, similarly to bilaterians, GW182 in Nematostella is recruited to the miRNA repression complex via interaction with Argonaute proteins, and functions downstream to repress mRNA. Thus, our work suggests that this mechanism of miRNA-mediated silencing was already active in the last common ancestor of Cnidaria and Bilateria. Despite this remarkable mechanistic conservation Argonaute and GW182 differentially co-evolved in bilaterians and cnidarians seemingly leading to distinct interaction surfaces. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: GW182 ; HEK293 ; AP-MS ; LC-MSMS

Contact

Principal Investigators:
(in alphabetical order) 		Matthias Selbach, Proteome Dynamics Group, Max Delbrueck Center, Berlin, Germany, N/A
Submitting User: ccms

Publications

Mauri M, Kirchner M, Aharoni R, Ciolli Mattioli C, van den Bruck D, Gutkovitch N, Modepalli V, Selbach M, Moran Y, Chekulaeva M.
Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution.
Nucleic Acids Res. 2017 Jan 25;45(2):938-950. Epub 2016 Sep 6.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.