Description
Skin sampling has been conducted as described in the [Molecular cartography of the human skin surface in 3D Amina Bouslimani, Carla Porto, Christopher M. Rath, Mingxun Wang, Yurong Guo, Antonio Gonzalez, Donna Berg-Lyon, Gail Ackermann, Gitte Julie Moeller Christensen, Teruaki Nakatsuji, Lingjuan Zhang, Andrew W. Borkowski, Michael J. Meehan, Kathleen Dorrestein, Richard L. Gallo, Nuno Bandeira, Rob Knight, Theodore Alexandrov, and Pieter C. Dorrestein PNAS April 28, 2015 112 (17) E2120-E2129] by swabbing areas of skin with a cotton swab and extracting the swabs using ethanol. The samples were stored in -80 C freezer until analysis. The GC-MS analysis was carried out on a Thermo Scientific TRACE 1310 GC-MS [TG-5ms 5; length, 30 m; ID (inner diameter), 0.25 mm; film thickness column, 0.25 um] and a TSQ 8000 EVO mass spectrometer (Thermo Fisher Scientific), equipped with electron ionization (EI) source, equipped with robotic sampler system. 1uL aliquot of sample was injected into the GC inlet maintained at 250C. The GC protocol was as follows: starting temperature 40 C (hold of 0.1 min), 15 C/min oven ramp to 280 C (hold of 0.1 min), and a 3 min hold period to purge the column. The helium carrier gas was set to constant 2 mL/min flow, splitless injection mode was used throughout. The scanned m/z range in a single quadrupole was 35-350 Th with solvent delay of 4 minutes.
[doi:10.25345/C5MW9G]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: skin ; ethanol ; swab ; GC-MS
Contact
Principal Investigators:
(in alphabetical order)
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Pieter Dorrestein, UCSD, United States
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aaksenov
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Identification Results |
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GNPS content goes here (MSV000084378 [task=4122afea75ce4ca09681957c1587560f])
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Number of distinct conditions across all analyses (original submission and reanalyses)
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Distinct condition labels are counted across all files submitted in the "Metadata" category
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"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses)
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Distinct replicate labels are counted across all files submitted in the "Metadata" category
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"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses)
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The technical replicate count is defined as the maximum number of times any one distinct
combination of condition and biological replicate was analyzed across all files submitted in the
"Metadata" category. In the case of fractionated experiments, only the first fraction is
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"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically
remapped by MassIVE to proteins in the
SwissProt
human reference database.
"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and
reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original
submission and reanalyses) associated with this dataset.
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Number of distinct peptide sequences (including modified variants or peptidoforms) reported
across all analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all
analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison
across all analyses (original submission and reanalyses) associated with this dataset.
A protein is differentially abundant if its change in abundance across conditions is found
to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated
with statistical tests for differential abundance.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE.
It has been imported to MassIVE for reanalysis purposes, so its spectra data here may
consist solely of processed peak lists suitable for reanalysis with most software.