MassIVE MSV000079626

Partial Public PXD003904

Application of 193 nm UVPD for phosphoproteomic analysis of HeLa and HCC70

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Description

Application of 193 nm ultraviolet photodissociation (UVPD) for phosphoproteomics analysis of IMAC enriched HeLa and HCC70 lysates. HCC70 cells were either maintained in full media or serum starved for 6 hours prior to lysis. Each sample was analyzed by three UVPD and three HCD technical replicates using an Orbitrap Fusion mass spectrometer equipped with a Coherent 193 nm excimer laser. Using UVPD, a/x, b/y, and c/z ions were generated and improved phosphate retention was observed on product ions. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Phosphoproteomics, UVPD, HCD, HeLa, HCC70

Contact

Principal Investigators:
(in alphabetical order) 		Jennifer Brodbelt
Submitting User: mrrobinson

Publications

Robinson MR, Taliaferro JM, Dalby KN, Brodbelt JS.
193 nm Ultraviolet Photodissociation Mass Spectrometry for Phosphopeptide Characterization in the Positive and Negative Ion Modes.
J. Proteome Res. 2016 Aug 5;15(8):2739-48. Epub 2016 Jul 26.

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