Genus-wide proteomics analysis of cobra (Naja) venoms. For top-down analysis, venom samples were reduced with TCEP and measured via HPLC-MS/MS (Q-Exactive) . Spectrum-Protein matching was performed with TopPic1.1 against a genus wide translated transcriptome database and NCBI protein entries from the najas.
For bottom-up analysis, HPLC separated fraction of venom were dried down, reduced with triethylphosphine and alkylated with 2-iodoethanol. HPLC-MS/MS experiments were performed on normalflow UHPLC-QTOF system (AB Sciex 5600 TripleTOF). Data analysis (PSM) was performed ProteinPilot version 4.0 and the transcriptome derived protein sequence database.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Top-down Proteomics ; Top-down Venomics ; Naja ; Snake venom ; Venom Proteomics ; Cobra
Principal Investigators: (in alphabetical order) |
Daniel Petras, University of California San Diego, USA Eivind Undheim, The University of Queensland, Australia |
Submitting User: | daniel |
Kazandjian TD, Petras D, Robinson SD, van Thiel J, Greene HW, Arbuckle K, Barlow A, Carter DA, Wouters RM, Whiteley G, Wagstaff SC, Arias AS, Albulescu LO, Plettenberg Laing A, Hall C, Heap A, Penrhyn-Lowe S, McCabe CV, Ainsworth S, da Silva RR, Dorrestein PC, Richardson MK, Gutiérrez JM, Calvete JJ, Harrison RA, Vetter I, Undheim EAB, Wüster W, Casewell NR.
Convergent evolution of pain-inducing defensive venom components in spitting cobras.
Science. 2021 Jan 22;371(6527):386-390.
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