MassIVE MSV000098295

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GNPS - Photosynthetic metabolite used for nitrogen fixation

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Description

Using 13C bicarbonate labelling, metabolites from photosynthesis will be identified and depletion of metabolites after 1hour of photosynthesis inhibition will reveal metabolites used for nitrogen fixation. Bicarbonate will be added at the beginning of the day, and samples will be taken after 5hours. Cells will be treated with DCMU (photosynthesis inhibitor) at this 5h timepoint and left for an additional 1h incubation. Final samples will be taken at 6h (1 hour after DCMU addition) to assess depletion of the photosynthetic metabolites. The work (proposal:https://doi.org/10.46936/10.25585/60008462) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231. [doi:10.25345/C5KS6JH5R] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: 13C labelling ; photosynthesis ; depletion ; DatasetType:Metabolomics

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Principal Investigators:
(in alphabetical order) 		Ellen Yeh, Stanford, United States
Submitting User: bpbowen
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GNPS content goes here (MSV000098295 [task=47196ebe973e4715b6a832ac38816e9b])
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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