MassIVE MSV000100274

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Bacteria covalently incorporate polyfluoroalkyl carboxylates into membrane lipids - Lipidomic Data

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Description

Background: Per- and polyfluoroalkyl substances (PFASs) are global contaminants of concern to human and environmental health. Little is known about biological attenuation processes and microbiota - PFAS interactions. Here we show that bacteria covalently incorporate n:3 polyfluoroalkyl carboxylates (FTCAs), including 5:3, 7:3, 8:3, and 9:3 FTCAs into phosphatidylethanolamine (PE) and phosphatidylglycerol (PG), two prominent components of lipid bilayers. When Pseudomonas sp. strain 273 was grown in the presence of 7:3 FTCA or 8:3 FTCA, semi-quantitative lipidomic analysis estimated that 7-12% of the bacteriums phospholipid pool contained the respective polyfluoroacyl chains, resulting in PFAS membrane incorporation factors (MIFs) of 423 plus-or-minus 92 and 686 plus-or-minus 127, respectively. Synthesis of PE and PG species carrying a polyfluorocarbon acyl tail was observed in five other axenic bacterial cultures tested, albeit with lower MIFs. Our results suggest that the formation of fluoromembranes is a prevalent bacterial process potentially impacting the fate, longevity, bioaccumulation, and biomagnification of PFASs. [doi:10.25345/C53T9DM05] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Perfluoroalkyl substances ; Polyfluoroalkyl substances ; PFAS ; Lipids ; Phospholipids ; Polyfluoroalkyl carboxylates ; FTCA ; LCMS ; Lipidomics ; DatasetType:Metabolomics

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Principal Investigators:
(in alphabetical order) 		Dr. Robert Hettich, Oak Ridge National Laboratory, the United States of America
Submitting User: ajstai
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