MassIVE MSV000097882

Partial Public PXD063902

Beta-cell Galphas signaling is critical for physiological and pharmacological enhancement of insulin secretion

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Description

Islet lysates were adjusted to 5 percent SDS in TEAB, pH 8.5, reduced and alkylated and digested with modified trypsin (Worthington) using an S-trap mini device (Protifi). Peptides were labeled with TMT16 reagents (lot ZH350094). Phosphopeptide enrichment used GL Sciences p10 TiO tips. LC-MS/MS used a MClass UPLC system (Waters) and Exploris 480 (ThermoFisher). Analytical separation used HSS T3 C18 75 micron by 250 mm column (Waters) with a 90 min gradient of 5 to 30 percent MeCN at a flow rate of 400 nl per min. Data collection on TMT-labeled samples was performed in data-dependent acquisition (DDA) mode with 2 FAIMS compensation voltages. TMT data was analyzed using Fragpipe 19. [doi:10.25345/C5QV3CG6H] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: IBMX ; phosphorylation ; islet ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Jonathan Campbell, Duke University, USA
Submitting User: mwfoster

Publications

Walejko JM, Christopher BA, Crown SB, Zhang GF, Pickar-Oliver A, Yoneshiro T, Foster MW, Page S, van Vliet S, Ilkayeva O, Muehlbauer MJ, Carson MW, Brozinick JT, Hammond CD, Gimeno RE, Moseley MA, Kajimura S, Gersbach CA, Newgard CB, White PJ, McGarrah RW.
Branched-chain ?-ketoacids are preferentially reaminated and activate protein synthesis in the heart.
Nat Commun. 2021 Mar 15;12(1):1680. Epub 2021 Mar 15.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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