MassIVE MSV000093624

Partial Public PXD047676

Phosphoproteomics of dried blood collected on Mitra devices

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Description

Venous blood was collected in Li-heparin or EDTA blood tubes and loaded onto 20 uL Mitra devices or separated to plasma as detailed in metadata. Samples were denatured in 5.5% SDC, reduced and alkylated, and digested with TPCK-trypsin followed by acid precipitation. 1 mg of filtered precipitates were enriched for phosphopeptides using TiO. Data was acquied using nanoLC, Evosep One or SPE-ZipChip. Data was acquired by DIA (Exploris 480 or TimsTOF Pro 2) or LFQ-DDA. DIA data was analyzed with Spectronaut 18 and DDA with Skyline. [doi:10.25345/C5028PQ5B] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: phosphorylation ; data-independent acquisition

Contact

Principal Investigators:
(in alphabetical order) 		Matthew W. Foster, Duke University, USA
Submitting User: mwfoster
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Identification Results
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Quantification Results
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
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