MassIVE MSV000088484

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A CpxA Phosphatase Inhibitor Activates CpxRA and is an Effective Treatment for Uropathogenic Escherichia coli in a Murine Model of Infection

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Description

For label-free quantitative proteomics, cell pellets from 5 independent experiments were subjected for protein extraction followed by LC-MS/MS. LC-MS/MS analysis was performed on a Dionex UltiMate 3000 (Thermo Fisher Scientific) system connected to a Q-Exactive HF-X mass spectrometer (Thermo Fisher Scientific). Peptide spectrum matching of MS/MS spectra was searched against the UniProt E. coli CFT073-UPEC database (TaxID:199310) using the Sequest algorithm within Proteome Discoverer v 2.4 software (Thermo Fisher Scientific). The Sequest database search was performed with the following parameters: trypsin enzyme cleavage specificity, 2 possible missed cleavages, 10 ppm mass tolerance for precursor ions, 0.02 Da mass tolerance for fragment ions. Search parameters permitted dynamic modification of methionine oxidation (+15.9949 Da) and static modification of carbamidomethylation (+57.0215 Da) on cysteine. Peptide assignments from the database search were filtered to a 1% False Discovery Rate. The relative label-free quantitative and comparative among the samples were performed using the Minora algorithm and Proteome Discoverer 2.4 software. Differential abundance of proteins between conditions was defined as a 1.5-fold change in relative abundance with an adjusted p-value < 0.05. [doi:10.25345/C5SW0M] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Escherichia coli, UPEC, CpxRA, phosphatase inhibitor, treatment

Contact

Principal Investigators:
(in alphabetical order) 		Stanley M Spinola, Indiana University, USA
Submitting User: kfortney
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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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