Domain level interactions between the ISWI remodeler SNF2h and reconstituted mononucleosomes were probed by EDC crosslinking under different biochemical conditions: ADP, ADP-BeFx, ADP-BeFx with LANA peptide added (which binds at the acidic patch). A semi quantitative approach was used to monitor domain-domain interactions enriched during the remodeling cycle.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: quantitative Crosslinking
Principal Investigators: (in alphabetical order) |
Geeta Narlikar, University of California San Francisco, USA |
Submitting User: | mtrnka |
Gamarra N, Johnson SL, Trnka MJ, Burlingame AL, Narlikar GJ.
The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h.
Elife. Epub 2018 Apr 17.
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