MassIVE MSV000079280

Partial Public PXD002816

Differential stoichiometry among core ribosomal proteins

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Description

Understanding the regulation and structure of ribosomes is essential to understanding protein synthesis and its dysregulation in disease. While ribosomes are believed to have a fixed stoichiometry among their core ribosomal proteins (RPs), some experiments suggest a more variable composition. Testing such variability requires direct and precise quantification of RPs. We used mass-spectrometry to directly quantify RPs across monosomes and polysomes of mouse embryonic stem cells (ESC) and budding yeast. Our data show that the stoichiometry among core RPs in wild-type yeast cells and ESC depends both on the growth conditions and on the number of ribosomes bound per mRNA. Furthermore, we find that the fitness of cells with a deleted RP-gene is inversely proportional to the enrichment of the corresponding RP in polysomes. Together, our findings support the existence of ribosomes with distinct protein composition and physiological function. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: ribosomes

Contact

Principal Investigators:
(in alphabetical order) 		Nikolai Slavov
Submitting User: nnslavov

Publications

Slavov N, Semrau S, Airoldi E, Budnik B, van Oudenaarden A.
Differential Stoichiometry among Core Ribosomal Proteins.
Cell Rep. 2015 Nov 3;13(5):865-73. Epub 2015 Oct 22.

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