MassIVE MSV000098982

Partial Public PXD067897

Li et al., Xylosyltransferase Bump-and-hole Engineering to Chemically Manipulate Proteoglycans in Mammalian Cells: Decorin glycosylated in vitro with 6AzGlc and clicked with ITag

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Description

Human FLAG-tagged decorin was overexpressed in CHO pgsA-745 cells and purified. Decorin was in vitro glycosylated with BH-engineered XT1 and UDP-6AzGlc, then clicked with ITag-alkyne, digested with trypsin on S-Trap, then analysed by HCD on an Orbitrap Eclipse. Data analysis with Byonic using 6AzGlc-ITag as a custom mod. [doi:10.25345/C5VX06G5R] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Glycosaminoglycan, chemical proteomics, glycosylation, glycosyltransterase ; DatasetType:Proteomics ; DatasetType:Other (Glycoproteomics)

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Principal Investigators:
(in alphabetical order) 		Benjamin Schumann, TUD Dresden University of Technology, Germany
Submitting User: bschumann
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