MassIVE MSV000088375

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Proteomic and single-cell transcriptomic dissection of human plasmacytoid dendritic cell response to influenza virus

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Description

Plasmacytoid dendritic cells [pDCs] represent a rare innate immune subset uniquely endowed with the capacity to produce substantial amounts of type-I interferons [IFN-I]. This function of pDCs is critical for effective antiviral defenses and has been implicated in autoimmunity. While IFN-I and select cytokines have been recognized as pDC secreted products, a comprehensive agnostic profiling of the pDC secretome in response to a physiologic stimulus has not been reported. We applied LC-MS/MS to catalogue the repertoire of proteins secreted by pDCs in response to challenge with live influenza H1N1. Additionally, using single-cell RNA-seq [scRNA-seq], we perform multidimensional analyses of pDC transcriptional diversification following stimulation. Our data reveal an abundance of protein species released by pDCs in addition to IFN-I, and evidence highly specialized roles within the pDC population ranging from dedicated cytokine super-producers to cells with APC-like functions. Moreover, dynamic expression of transcription factors and surface markers characterize activated pDC fates. [doi:10.25345/C5887D] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: H1N1 ; Influenza ; pDC

Contact

Principal Investigators:
(in alphabetical order) 		Peter Gregersen, Feinstein Institutes for Medical Research, USA
Submitting User: proteomics2_columbia

Publications

Ghanem MH, Shih AJ, Khalili H, Werth EG, Chakrabarty JK, Brown LM, Simpfendorfer KR, Gregersen PK.
Proteomic and Single-Cell Transcriptomic Dissection of Human Plasmacytoid Dendritic Cell Response to Influenza Virus.
Front Immunol. Epub 2022 Mar 23.

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