MassIVE MSV000084938

Imported Reanalysis Dataset Public PXD001293

Comaprison of the phagosome proteome of bone marrow-derived macrophages and RAW264.7

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Description

Macrophages are important immune cells operating at the forefront of innate immunity by taking up foreign particles and microbes through phagocytosis. The RAW 264.7 cell line is commonly used for experiments in the macrophage and phagocytosis field. However, little is known how its functions compare to primary macrophages. Here, we have performed an in-depth proteomics characterisation of phagosomes from RAW 264.7 and bone marrow-derived macrophages by quantifying more than 2500 phagosomal proteins. Our data indicates that there are significant differences for a large number of proteins including important receptors such as mannose receptor 1 and Siglec-1. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: macrophage ; phagosome ; mouse ; RAW264.7 ; MassIVE.quant reviewed - Gold

Contact

Principal Investigators:
(in alphabetical order) 		Matthias Trost, MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, DD1 5EH, Scotland, United Kingdom., N/A
Submitting User: ccms

Publications

Guo M, Härtlova A, Dill BD, Prescott AR, Gierli?ski M, Trost M.
High-resolution quantitative proteome analysis reveals substantial differences between phagosomes of RAW 264.7 and bone marrow derived macrophages.
Proteomics. 2015 Sep;15(18):3169-74. Epub 2015 Feb 5.

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