MassIVE MSV000083034

Partial Public PXD011363

integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize

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Description

Glyphosate tolerant genetically modified (GM) maize NK603 was assessed as substantially equivalent to its isogenic counterpart by a nutrient composition analysis in order to be granted market approval. We have applied contemporary in depth molecular profiling methods of NK603 maize kernels (sprayed or unsprayed with Roundup) and the isogenic corn to reassess its substantial equivalence status. Proteome profiles of the maize kernels revealed alterations in the levels of enzymes of glycolysis and TCA cycle pathways, which were reflective of an imbalance in energy metabolism. Changes in proteins and metabolites of glutathione metabolism were indicative of increased oxidative stress. The most pronounced metabolome differences between NK603 and its isogenic counterpart consisted of an increase in polyamines including N-acetyl-cadaverine (2.9-fold), N-acetylputrescine (1.8-fold), putrescine (2.7-fold) and cadaverine (28-fold), which depending on context can be either protective or a cause of toxicity. Our molecular profiling results show that NK603 and its isogenic control are not substantially equivalent. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: maize

Contact

Principal Investigators:
(in alphabetical order) 		Michael Antoniou, King's College London, UK
Submitting User: MA_group

Publications

Mesnage R, Agapito-Tenfen SZ, Vilperte V, Renney G, Ward M, Séralini GE, Nodari RO, Antoniou MN.
An integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize reveals metabolism disturbances caused by the transformation process.
Sci Rep. Epub 2016 Dec 19.

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