Identification of phosphopeptides EZH2 in control and kinase-deficient cells.
The HA-EZH2 plasmid was transfected in HEK 293T cells, and HA-EZH2 was subsequently purified using HA peptide. The elution was performed with 1mg/mL HA-peptide in Tris buffered saline (TBS with 150mM NaCl), pH 7.5 in a final volume of 100 microL and a concentration of 150-250 ng/microL. Two samples were sent to the facility for analysis: HEK293T-sgC and sgPRKCI.
[doi:10.25345/C5639KH0K]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: EZH2 ; PRKCI ; Prostate Cancer
Principal Investigators: (in alphabetical order) |
Maria T. Diaz-Meco Conde, WEILL CORNELL MEDICINE, United States |
Submitting User: | moscatdiazme |
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