MassIVE MSV000093438

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Membrane Proteome Profiling of Erythroid Cell Lineages: A Valuable Resource for Advancing Plasmodium vivax Research

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Description

Background: Erythropoiesis is an intricate maturation process culminating in the production of erythrocytes. As cells progress through erythropoiesis, they undergo surface loss, organelle expulsion, size reduction, and dynamic removal of membrane proteins, refining their structure for specialized physiological functions. This orchestrated process is essential for generating mature erythrocytes capable of efficient gas exchange in the circulatory system. The membrane composition of red blood cells, comprising lipid layers and embedded proteins that play a fundamental role in cellular stability, flexibility, and host interactions. Investigating the membrane proteome becomes particularly relevant in the context of infectious diseases, such as malaria caused by Plasmodium. These proteins critically interact with parasite ligands, allowing entry and progression through various life cycle stages within host cells. Among Plasmodium species, P. vivax exhibits a specific tropism for invading reticulocytes, posing challenges for in vitro continue cultures development due to their limited availability and rapid differentiation. To address this, we studied hematopoietic cell lines, JK-1 and BEL-A2, as potential alternatives to reticulocytes for studying P. vivax biology. Using a Data Independent Acquisition (DIA) LC-MS/MS approach, we characterize and compare the membrane proteomes of reticulocytes, erythrocytes, and these cell lines. Results: Our results reveal distinct abundance patterns in transmembrane proteins, skeletal proteins, and membrane transporters during erythropoiesis. Additionally, we identified 6 potential receptor candidates for P. vivax merozoites ligands, shedding light on the intricate host-parasite interactions. Furthermore, we detected the loss of membrane proteins during erythroblast enucleation, providing insights into the cellular processes governing this transition. Conclusions: This study significantly contributes to our understanding of erythropoiesis, offering valuable insights for P. vivax malaria research and in vitro culture system development. The comprehensive analysis of membrane proteomes in different cell stages establishes a foundation for future investigations into cellular homeostasis, disease mechanisms, and potential therapeutic targets. [doi:10.25345/C5W669K65] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Malaria ; Plasmodium vivax ; red blood cells ; erythroid cell lines ; reticulocytes

Contact

Principal Investigators:
(in alphabetical order) 		Manuel Alfonso Patarroyo, Fundacion Instituto de Inmunologia de Colombia, Colombia
Markus Kalkum, City of Hope, United States
Submitting User: jessicamolina
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