We aim to determine the physiological impact of inactivating E2F in muscles and in adipose cells in vivo. To uncover the mechanism of action of E2F in each tissue we investigated the similarities between E2F-deficient tissues by comparing the proteomic profiles between muscles and fat cells. Our findings revealed that E2F is required in fat cells to promote the storage of fat and the synthesis of trehalose, which was restored by feeding animals in high sugar diet.
Multiplexed quantitative proteomics was performed using TMT10-plex reagents on an Orbitrap Fusion mass spectrometer using the SPS-MS3 method.
Two sample sets - fatbody samples and samples of thoracic muscles - were analyzed using two TMT sets. Twelve high pH reversed-phase chromatography fractions were analyzed per sample set.
The samples in each set were TMT-labeled as follows:
Fatbody samples:
WT FB MS1; 126
DP FB MS 1; 127n
WT FB MS2; 127c
DP FB MS 2; 128n
WT FB MS3; 128c
DP FB MS 3; 129n
Thoracic muscles samples:
Mef>Ci 1; 126
Mef>Dpi 1; 127n
Mef>Ci 2; 127c
Mef>Dpi 2; 128n
Mef>Ci 3; 128c
Mef>Dpi 3; 129n
Mef>Ci 4; 129c
Mef>Dpi 4; 130n
[doi:10.25345/C5R494]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: drosophila melanogaster, E2F/Dp, Orbitrap Fusion, TMT10, SPS-MS3
Principal Investigators: (in alphabetical order) |
Maxim V Frolov, University of Illinois at Chicago, United States |
Submitting User: | whaas |
Zappia MP, Guarner A, Kellie-Smith N, Rogers A, Morris R, Nicolay B, Boukhali M, Haas W, Dyson NJ, Frolov MV.
E2F/Dp inactivation in fat body cells triggers systemic metabolic changes.
Elife. Epub 2021 07 12.
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