MassIVE MSV000088979

Partial Public PXD032026

Honey proteome of the bumblebee Bombus terrestris

Description

The biological samples analyzed in this study were honey samples from the bumblebee B. terrestris. Commercially available Tripol hives used for pollination consist of three complete bumblebee colonies (Koppert Biological Systems). Four Tripol hives (12 colonies overall) were placed in the Crop Research Institute (Prague-Ruzyne, Czechia) at the time of rapeseed Brassica napus and apple tree flowering. Honey stores were manually collected into 50-mL sterile centrifuge tubes. Honey samples per colony were pooled. These 12 honey samples were analyzed using label-free nano-LC-MS/MS. In addition, 3 bands excised from 1D-E SDS-PAGE were analyzed using nano-LC-MS/MS. Note that modification for analysis the bands was carbamidomethyl instead of MethylThio. Combined.txt and used databases are provided. [doi:10.25345/C54J09Z9W] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: honey ; honey proteins

Contact

Principal Investigators:
(in alphabetical order)
Tomas Erban, Crop Research Institute, Czechia
Submitting User: arachnid
Number of Files:
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Experimental Design
    Conditions:
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Identification Results
    Proteins (Human, Remapped):
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Quantification Results
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.