MassIVE MSV000086780

Partial Public PXD023895

Exosome miRNA protein interactions in brown adipocytes

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Description

We have identified several nucleotide motifs (caug, cgggag=S2) that promote exosome sorting of miRNA in different cell types including brown adipocytes. In order to identify which proteins might recognize and bind to these motifs, we have performed co-precipitations of proteins binding biotinylated forms of miRNAs containing the aforementioned motifs or none - using streptavidin beads incubated with brown adipocytes cell lysates. We have included two types of controls: negative poly-A control and a scramble miRNA. [doi:10.25345/C55N5H] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: exosomes ; microRNA ; data-independent acquisition ; interaction ; adipocytes

Contact

Principal Investigators:
(in alphabetical order) 		Birgit Schilling, Buck Institute for Research on Aging, United States
Submitting User: bschilling
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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