MassIVE MSV000087080

Partial Public PXD024885

Molecular physiological characterization of a high heat resistant spore forming Bacillus subtilis food isolate

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Description

Bacterial endospores (spores) are among the most resistant living forms on earth. Spores of Bacillus subtilis A163 show extremely high resistance to wet heat compared to spores of laboratory strains. In this study, we found that spores of B. subtilis A163 were indeed very wet heat resistant and released dipicolinic acid (DPA) very slowly during heat treatment. We also determined the proteome of vegetative cells and spores of B. subtilis A163 and the differences in these proteomes from those of the laboratory strain PY79, spores of which are much less heat resistant. This proteomic characterization identified 2011 proteins in spores and 1901 proteins in vegetative cells of B. subtilis A163. [doi:10.25345/C5ZN5N] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Proteomics ; Microbiology ; Bacillus subtilis ; timsTOF pro ; label free quantitation ; strain A375 ; strain P79

Contact

Principal Investigators:
(in alphabetical order) 		Gertjan Kramer, University of Amsterdam, Netherlands
Submitting User: gkramer

Publications

Zhiwei Tu, Peter Setlow, Stanley Brul, Gertjan Kramer.
Molecular Physiological Characterization of a High Heat Resistant Spore Forming Bacillus subtilis Food Isolate.
Microorganisms . 2021 Mar 23;9(3):667.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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