Description
In neurons it is assumed mitochondrial replication only occurs in the cell body and then mitochondria have to travel to the periphery. Although mitochondrial replication has been observed to occur away from the cell body, the mechanisms involved are still elusive. Using EdU-labelling in mouse primary neurons, we developed a tool to determine the mitochondrial replication rate. Taking of advantage of microfluidic devices, we confirmed that mitochondrial replication also occurs locally in the periphery of neurons. To achieve this, mitochondria require de novo nuclear-encoded, but not mitochondrial-encoded protein translation. Following a proteomic screen comparing synaptic with non-synaptic mitochondria we identified two elongation factors eEF1A1 and TUFM that were upregulated in synaptic mitochondria. We found that mitochondrial replication is impaired upon downregulation of eEF1A1, and this is particularly relevant in the periphery of neurons.
[doi:10.25345/C52B8VN9T]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Mitochondria ; Synapse
Contact
Principal Investigators:
(in alphabetical order)
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Jeffrey N. Savas, PhD, Department of Neurology Northwestern University, USA
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jeffsavas
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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
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