Spotted 10uM of phenylacetylglutamine on a metal surface and used wooden cotton swabs to wipe the area after 45min. Extracted the recovery in Eppendorfs through centrifugation. Dried and resuspended the samples in 50% methanol spiked with sulphadimethoxine for LC-MS analysis. Spotted samples were wiped for 1min vs 5sec, once vs twice, and the cotton swab rinsed in some cases. Data file names in the master sequence and Skyline report are labeled as "car-XX.raw", but in all cases the standard is actually phenylacetylglutamine.
[doi:10.25345/C59582]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: mass spectrometry ; Phenylacetylglutamine
Principal Investigators: (in alphabetical order) |
Dr. McCall, OU, US |
Submitting User: | narcissubox |
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